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    当前位置:首页资料下载兔抗钥孔戚血蓝素IgGRabbit Anti KLH IgG ELISA 说明书

    兔抗钥孔戚血蓝素IgGRabbit Anti KLH IgG ELISA 说明书

    发布时间:2023/11/27点击次数:925

    兔抗钥孔戚血蓝素IgG检测试剂盒

    Rabbit Anti Keyhole limpet hemocyanin (KLH) IgG ELISA Kit

    cat#700-110-KLR

    INTENDED USE

    The Rabbit Anti-Keyhole Limpet Hemocyanin (KLH) ELISA Kit is an immunoassay

    suitable for quantifying or titering total antibody activity (IgG, IgA and IgM) specific

    for KLH in serum, plasma or other biological fluids. For research use only (RUO), not for

    diagnosis, cure or prevention of the disease.

    RESEARCH USE OF THE TEST

    Keyhole Limpet Hemocyanin (KLH), an oxygen-transporting protein of the

    marine gastropod Megathura crenulata, is recognized as a potent

    immunoactivator, and therefore is widely used in research and clinical

    studies. Present applications of KLH include: (a) use as a highly

    immunogenic antigen for assessment of immune competence of an

    organism, and (b) frequent use as a carrier of low molecular mass peptides

    and haptens, such as oligosaccharides, gangliosides or (glyco)peptides, designed to facilitate

    antibody production. In these cases, antibodies made to small peptides/haptens are generally

    raised by coupling to a large carrier protein like KLH. Antibodies are produced to both KLH and

    the peptide/hapten; because anti-KLH may give non-specific signals in various immunoassays,

    removal by solid phase immunoaffinity chromatography is common. The ELISA is useful for

    determining levels of anti-KLH in sera and for monitoring anti-KLH removal from purified samples

    after antibody production.

    The ADI anti-KLH ELISA is designed with high sensitivity for discriminating the lower level

    antibodies, with specially formulated diluents to minimize interfering background signals.

    PRINCIPLE OF THE TEST

    The Rabbit KLH ELISA kit is based on the binding of rabbit anti- KLH

    in samples to KLH immobilized on the microwells, and anti- KLH Ig

    antibody is detected by anti-rabbit Ig-specific antibody conjugated to

    HRP (horseradish peroxidase) enzyme. After a washing step,

    chromogenic substrate (TMB) is added and color is developed by the

    enzymatic reaction of HRP on the substrate, which is directly

    proportional to the amount of anti-KLH present in the sample.

    Stopping Solution is added to terminate the reaction, and absorbance

    at 450nm is then measured using an ELISA microwell reader. The

    amount of rabbit Ig in samples is calculated relative to rabbit anti-KLH reference calibrators.

    Specificity

    Purified KLH is used to coat the microwells; thus the assay is specific for antibodies

    directed to KLH. The anti-rabbit IgG (H+L) HRP conjugate reacts with rabbit IgG, IgA

    and IgM class antibodies that bind to KLH on the plate. IgE antibody would not be

    measured above background signals.

    Assay Sensitivity

    The KLH coating level and HRP conjugate concentration are optimized to differentiate

    anti-KLH Ig from background (non-antibody) signal with rabbit serum samples diluted

    1:100.

    Calibrator Values

    The calibrators are dilutions of rabbit IgG antibody reactive to KLH. Values are

    assigned as arbitrary Anti-KLH activity units (see Limits of the Assay).


    更多产品详情,请联系 Alpha Diagnostic 中国区总代理北京博蕾德生物科技有限公司。


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